How Mesenchymal Stem Cells are Harvested

Where They Are Found In Your Body and How We Harvest and Use Them For Therapy.

How We Harvest Millions
of Your Live Mesenchymal Stem Cells

How Mesenchymal Stem Cells are Harvested

Many stem cell therapy providers still harvest mesenchymal stem cells from bone marrow which involves drilling a hole usually in the patient’s hip bone and using a syringe to extract bone marrow. Most of the stem cells found in bone marrow are hematopoietic stem cells that become red blood cells, platelets, or white blood cells. Mesenchymal stem cell counts in bone marrow are less than 10,000 cells per 2 ounces of bone marrow.

Purchasing and using cryopreserved, (frozen) umbilical cord stem cells is another popular option with many stem cell therapy providers. However, there is a possibility of genetic and epigenetic changes to occur in cryopreserved cells and the freezing and thawing process itself can lead to low cell survival rates

At Pangenics Regenerative Center our stem cell physicians use a proprietary 38-step process to prepare millions of live mesenchymal stem cells harvested from a patient’s adipose tissue, (body fat). This method of harvesting eliminates any possible issues with genetics, greatly reduces or eliminates recovery times and produces up to 50 million live, viable mesenchymal stem cells that can be immediately injected into the areas targeted for therapy.

The Best Source of Mesenchymal Stem Cells

Stem cell therapy begins with local anesthesia in the areas where our stem cell physicians will use the Adivive enclosed fat transfer system’s liposuction to collect a small amount of body fat. More specifically “stromal fat”, or SVF, (Stromal Vascular Fraction). This area of body fat is rich with blood vessels where mesenchymal stem cells are stored by the body in very large quantities. Patients then simply relax while the SVF is processed and prepared to be transferred to the therapy target areas.

Stromal Vascular Fraction, SVF Explained

Stromal: Best explained as a pinch of fat. The first layer of fat directly beneath the skin.

Vascular: Within the Stromal fat, there are tiny blood vessels where mesenchymal stem cells are found in abundance.

Fraction: The heterogeneous group of cells derived from the fat that contains mesenchymal progenitor/stem cells, preadipocytes, endothelial cells, pericytes, T cells, and M2 macrophages.

The SVF is processed through a series of steps including centrifuge that separates oils and digests the fat with an enzyme solution. The fat and enzymes are then incubated and mixed. The collagenase is centrifuged again to isolate and remove fat cells. The collagenase is rinsed from the remaining SVF three times. And finally, the SVF is then put through a 100 micron filter to produce 8cc to 12cc of SVF containing up to 50 million mesenchymal stem cells. Quantities vary depending on the patient.

Also during the preparation of the patient’s SVF, the Magellan® Autologous Platelet Separator uses a small amount of the patient’s blood to produce Platelet Rich Plasma, (PRP). PRP contains platelets and seven different growth factors that will be mixed with the prepared SVF to nourish the mesenchymal stem cells and help them survive the transfer and flourish.

In order to accurately document results, we also use a NucleoCounter® Cell Counter to count the number of live mesenchymal stem cells that were successfully harvested and also to asses their viability, (health and likelihood of survival). This data along with other details of the therapy are documented in a national IRB to advance the study and further research on the medical use of mesenchymal stem cells. 

Magellen Platelet Separator

Magellen Platelet Separator

The Magellan Platelet Separator is used to produce platelet rich plasma, (PRP) from a small amount of the patient’s blood. The Magellan Platelet separator filters the blood to collect platelets and seven different growth factors from the patient’s blood which is them mixed with the patient’s mesenchymal stem cells. This step helps the mesenchymal stem cells survive, multiply and become replacement cells.

NucleoCounter NC-200 Cell Counter

NucleoCounter NC-200 Stem Cell Counter

The NucleoCounter Stem Cell Counter is used identify the total number of Live, Viable Mesenchymal Stem Cells before they are used in therapy. The exact number of live, viable stem cells harvested from each patient and used for their stem cell therapy is very important and is documented in the IRBs we are participating in.

Millions of Live, Viable Mesenchymal Stem Cells Verified

The mesenchymal stem cell count below is from a patient’s records. During this patient’s therapy, we successfully harvested over 17 million live mesenchymal stem cells from only 2 ounces of the patient’s body fat.

Viable Stem Cell Confirmation Counts

How Stem Cell Therapy is Performed

The final step of stem cell therapy can now be performed. Millions of your own live, viable mesenchymal stem cells have been collected and isolated. They have been counted to verify needed quantities, and they have been nourished with platelets and seven different growth factors, (PRP). Millions of your stem cells are now ready to do their job and are injected directly into the joints targeted for therapy and also administered intravenously to maximize the therapy. Once the injections and or IV has been administered, the therapy is complete. There are no side effects and patients are able to drive home or back to work shortly after their therapy.